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Malaysian Journal of Analytical Sciences, Volume 20, Issue 6, 2016, pp. 1233-1240

Studies of interaction between tetrabutylammonium bromide based deep eutectic solvent and DNA using fluorescence quenching method and circular dichroism spectroscopy

Abstract :

The interaction of tetrabutylammonium bromide (TBABr)-based deep eutectic solvents (DESs) with calf thymus DNA (ct-DNA) was investigated by fluorescence and circular dichroism (CD) spectroscopy. The influences of hydrogen bond donors (HBDs), with respect to chain lengths and the presence of OH group were determined through ethidium bromide (EB) fluorescence quenching. Based on the quenching process, it was concluded that hydrophobic interactions were responsible for the DES ability to quench EB from EB-bound DNA. The strong electrostatic attraction between TBA+ cation on DES and negative charge phosphate DNA was proved from fluorescence and CD spectroscopy. DNA was able to maintain its double helical structure at 25 °C with a concentration of 25% DES. © 2016, Malaysian Society of Analytical Sciences. All rights reserved.

Keywords : Binding,Circular dichroism spectroscopy,Deep eutectic solvent,Fluorescence quenching,Tetrabutylammonium bromide
Subject Area : Analytical Chemistry

Kajian Interaksi antara Pelarut Eutektik Mendalam Berasaskan Tetrabutilamonium Bromida dan DNA Menggunakan Kaedah Pelindapkejutan Pendarfluor dan Spektroskopi Edaran Dikroisme

Abstract :
Keywords :
Subject Area : Analytical Chemistry

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